Figure 1

(A) Expression of transcripts in mouse lung tissue in response to PVM infection: IFN-γ and IFN-γ correlating profiles. Baseline expression in uninfected mice (day 0) is set at 1.0 and normalized expression (per gene, per chip) is shown for days 1 – 7, 10, 14, 21 and 28 after inoculation. Profiles of 203 transcripts with patterns that correlate with that the profile of IFN-γ (0.900 to 0.969) are shown in yellow to red, respectively, and identified by name in Table 1. The expression profile of CCL3 (MIP-1α), a chemokine crucial for neutrophil recruitment in response to PVM infection, is overdrawn with a blue line (correlation 0.965). (B) Correlation of IFN-γ and CCL3 protein levels in individual PVM-infected mice. IFN-γ and CCL3 detected by ELISA in lung tissue homogenates from individual mice days 0 – 28 after inoculation with 30 pfu PVM (n = 43) are as shown. (C) IFNγ⁺ NK and T cells detected in lungs of PVM-infected mice. Total and IFNγ⁺ NK cells, CD4⁺ T cells, and CD8⁺ T cells (± sd) detected per lung on day 6 after inoculation with 10 pfu PVM.