Figure 1

The chicken immune response as monitored by detection of antigen-specific IgY. (A) ELISA: chicken sera were tested at 10-fold dilution steps starting from a 1:100 dilution. The serum before immunization was used as the negative control. Each line represents the mean absorbance of 5 individual chicken sera ± standard deviation. (B) Competition assay for polyclonal chicken sera: for the competition assay, V5B2 was pre-incubated with the chicken sera that had been previously immunized with V5B2, and the subsequent binding ability of V5B2 to the P1 peptide was measured by ELISA. Each column represents the mean inhibition of V5B2 binding to the P1 peptide from the 4 individual chicken sera with the highest antibody titres ± standard deviation. (C) Competition assay for immunity purified chicken IgY (Ch5): each column represents the mean inhibition of V5B2 binding to the P1 peptide of at least three experiments ± standard deviation.