Figure 3

Lis1 protein mislocalised in Nrg mutants. (A) Haemocytes were bled from wandering third instar control (w¹¹¹⁸), Nrg^{G 00305}, or UAS-Nrg^IR;He-Gal4 larvae and stained for Lis1 expression (red) and actin (green), nuclei were visualised by DAPI staining (blue). In control cells Lis1 was observed tightly associated with the nucleus and at centriole-like structures also tightly associated with the nucleus (arrows). In plasmatocytes from Nrg^{G 00305}or UAS-Nrg^IR;He-Gal4 larvae Lis1 was more diffuse throughout the cytoplasm, and in many cells Lis1 expression was enhanced at centriole-like structures (arrowheads). (B) Lis1 perinuclear expression levels. Haemocytes were bled from control, Nrg^{G 00305}or UAS-Nrg^IR;He-Gal4 wandering third instar larvae. The haemocytes were stained for Lis1 expression. ImageJ was used to measure fluorescence intensity of Lis1 staining surrounding the nucleus of at least 75 haemocytes from three different larvae. Asterisks indicate a significant difference from control cells. (Student's t-test, P < 0.01).