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Table 2 The effect of pertussis toxin on TM-N49 induced histamine release from human mast cells

From: Induction of mast cell accumulation, histamine release and skin edema by N49 phospholipase A2

Compound

(μg/ml)

Net histamine release (%)

  

Without pertussis toxin

With pertussis toxin

  

Lung

Colon

Tonsil

Lung

Colon

Tonsil

TM-N49

3.0

9.3 ± 1.9

13.5 ± 3.0

8.0 ± 2.0

0.8 ± 1.8*

0.1 ± 2.2*

1.5 ± 1.3*

 

30

14.7 ± 4.7

29.6 ± 6.8

62.0 ± 8.5

4.0 ± 0.6*

3.9 ± 3.9*

31.9 ± 9.2*

Anti-IgE

10

17.3 ± 5.4

17.4 ± 5.1

11.1 ± 3.4

2.1 ± 1.1*

0.9 ± 1.2*

3.0 ± 0.3*

CI

0.5

24.4 ± 6.3

38.8 ± 4.6

37 ± 9.3

19.6 ± 4.8

30.4 ± 2.8

20.4 ± 4.4

  1. The values shown are mean ± SE for four to five separate experiments performed in duplicate. Cells were incubated with1.0 μg/ml pertussis toxin for 4 h at 37°C before challenging with stimulus. * P < 0.05 compared with the corresponding uninhibited controls.
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BMC Immunology

ISSN: 1471-2172

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