Figure 1

PA and immune cells cooperate in pancreatic cell viability reduction. RIN cells were treated with various doses of PA (A), or they were cultivated alone (medium) or co-cultivated with LNC (B, C) in the absence (B) or presence (C) of tissue culture inserts, or cultivated in the presence of 40% Sn (D) or 10% Sn (F), in the absence or presence of 125 μM PA (B-D) or 32 μM PA (F). Primary pancreatic islets were cultivated alone (medium) or in the presence of 40% Sn, in the absence or presence of 125 μM PA (E). MTT assay was performed after 20 hours of cultivation and the results are presented as the percentage of control absorbance values obtained for cultures grown in medium alone. Mean values +/- SD of values obtained in 8 (A), 14 (B), 3 (C), 11 (D), 3 (E) and 6 (F) individual experiments are presented. *p < 0.05 represents a statistically significant difference between values obtained from RIN cell cultures treated with PA in the presence of LNC and any other culture of RIN cells or RIN cells treated with PA and Sn and any other culture of RIN cells.