The expression of the β-defensins hBD-2 and hBD-3 is differentially regulated by NF-κB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis
© Steubesand et al; licensee BioMed Central Ltd. 2009
Received: 03 April 2009
Accepted: 12 June 2009
Published: 12 June 2009
Candida albicans resides on epithelial surfaces as part of the physiological microflora. However, under certain conditions it may cause life-threatening infections like Candida sepsis. Human β-defensins (hBDs) are critical components of host defense at mucosal surfaces and we have recently shown that hBD-2 and hBD-3 are upregulated in Candida esophagitis. We therefore studied the role of Candida te signalling pathways in order to understand the mechanisms involved in regulation of hBD-expression by C. albicans. We used the esophageal cell line OE21 and analysed the role of paracrine signals from polymorphonuclear leukocytes (PMN) in an in vitro model of esophageal candidiasis.
Supernatants of C. albicans or indirect coculture with C. albicans induces upregulation of hBD-2 and hBD-3 expression. PMNs strongly amplifies C. albicans- mediated induction of hBDs. By EMSA we demonstrate that C. albicans activates NF-κB and AP-1 in OE21 cells. Inhibition of these pathways revealed that hBD-2 expression is synergistically regulated by both NF-κB and AP-1. In contrast hBD-3 expression is independent of NF-κB and relies solely on an EGFR/MAPK/AP-1-dependent pathway.
Our analysis of signal transduction events demonstrate a functional interaction of epithelial cells with PMNs in response to Candida infection involving divergent signalling events that differentially govern hBD-2 and hBD-3 expression.
Candida albicans colonize distinct microanatomical regions such as the oro-gastointestinal tract as a commensal, but also accounts for more than 50% of all fungal systemic infections . Candida esophagitis represents a severe threat to an immunocompromised body and, especially in neutropenic patients, often is the first manifestation before Candida sepsis develops [2, 3]. Host defense mechanisms preventing mucosal (e.g. esophageal) candidiasis are poorly understood, but include both innate and adaptive responses [4, 5].
The gastrointestinal epithelial layer represents a barrier that is usually adequate to restrain commensal microbes, but is often insufficient to protect against microbial pathogens. Once this physical barrier is penetrated, recognition of invading microbiota is the first step in the initiation of a fast immune response and involves the activation of pattern recognition receptors by microbial pathogens and their products [6–8]. C. albicans have been shown to activate a subset of pattern recognition receptors, the Toll-like receptors (TLRs). This family of transmembrane receptors recognizes a broad variety of signature motifs on microbes and transduces signals leading to the activation of transcription factors , production of cytokines and antimicrobial peptides [10, 11]. However there is some controversy and inconclusive data which TLR subtypes are activated by C. albicans. Some reports indicated a critical role for TLR2 and TLR4 in activating the host defense response alone or in combination with the ss-glucan receptor Dectin-1,[5, 12, 13] whereas other studies showed that TLR-1 and TLR-6 are responsible for the recognition of C. albicans [14, 15].
In addition the downstream effectors in the immune response against C. albicans are largely unknown. There is growing evidence that human β-defensins (hBDs) are critical components of both the innate and adaptive immune responses to Candida infections with distinct antifungal efficacies and mechanisms for hBD-2 and hBD-3 [16–19]. Expression of hBDs is regulated by a plethora of proinflammatory cytokines like IL-1β, TNF-α and EGF-receptor ligands activating downstream effectors like the transcription factors NF-κB or AP-1 [20–22]. hBDs are secreted by epithelial cells and protect the gastrointestinal mucosa by antimicriobial activity as well as by chemotactic properties recruiting polymorphonuclear leukocytes (PMN) to the site of infection [21, 23]. Transepithelial migration of PMNs is observed during oral Candida infection and is believed to play a crucial role in the clearance of infection and in epithelial homeostasis [5, 24]. This protective phenotype is associated with production of epithelial proinflammatory cytokines, including IL-8, IL-1β and TNF-α. However, the mechanisms by which PMNs and epithelial cells interact to protect mucosal surfaces from C. albicans invasion are mostly unknown.
We have recently shown that hBD-2 and hBD-3 are highly expressed in Candida esophagitis and that the α-defensins Human Neutrophil Peptides 1–3 were also upregulated  indicating involvement of neutrophils in the immune response to the C. albicans infection . This is in line with the observation that IL-8 is strongly expressed in the mucosa of patients suffering by Candida infection , since this cytokine is involved in recruitment of polymorphonuclear leukocytes (PMNs) to sites of microbial infection .
In the present study we explored the mechanisms involved in the induction of hBD expression in an in vitro model of Candida esophagitis. Using this co-culture model we analyzed the contribution of PMNs in the regulation of epithelial hBD expression. We found that hBD-2 and hBD-3 are strongly upregulated through high concentrations of C. albicans alone. If lower concentrations of C. albicans were used, only a moderate induction of these hBDs were observed that was strongly enhanced by coculture of the epithelial cell line with PMNs, emphasizing the important role of mesenchymal-epithelial interactions in early host defense against fungal infection. Furthermore we were able to show that the NF-κB and MAPK signalling pathways contribute to the regulation of hBD-2 and that hBD-3 is a downstream target of a distinct EGFR/MAPK/AP-1 pathway in Candida esophagitis.
Supernatants of C. albicans induce hBD expression in OE21 cells
PMNs significantly enhances hBD-2 and hBD-3 expression during C. albicans infection
C. albicans activate NF-κB in OE21 cells
NF-κB is involved in the induction of hBD-2 but not hBD-3 expression
Role of AP-1 and the MAPK Pathway in C. albicans mediated β-defensin induction
Activation of the EGFR is an upstream event in regulation of hBD-3 expression
TGF-α is involved in EGFR mediated hBD-3 induction
Candida esophagitis represents a severe threat to an immunocompromised body and the course of the infection is determined by both pathogen- and host-dependent factors [15, 24, 38, 39]. It is well established that epithelial cells of the esophagus are the central target of an oro-esophageal invasive Candida infection but there are only very limited data on the host response preventing a Candida esophagitis. In the present study we identified the NF-κB and MAPK/AP-1 pathways as central regulators of epithelial hBD-2 and hBD-3 expression during C. albicans infection. Furthermore we were able to show a crucial role of the interaction of PMNs with the epithelial cell compartment for the induction of hBD-2 and hBD-3 expression during Candida infection of esophageal epithelial cells. Finally hBD-3 expression is dependent on transactivation of EGFR by TGF-α. This is the first report delineating molecular mechanisms leading to upregulation of hBD-2 and hBD-3 in esophageal Candida infection suggesting differential regulation of these two central epithelial antibiotic peptides in response to a C. albicans infection. We were able to show a concentration dependency of hBD-2 and hBD-3 induction upon stimulation with C. albicans supernatants (Figure 1B). This dose dependent effect could be at least in part be mediated through stronger induction of NF-κB (Figure 4) and AP-1 (Figure 6) through higher concentrations of C. albicans. A comparable concentration-dependent effect of supernatants of environmental airborne fungi on cytokine release of eosinophils  and of C. albicans on the activation of NF-κB  was recently reported.
There is some controversy about the relevance of the NF-κB and AP-1 binding sites in the promotor of hBD-2 for full induction of hBD-2 expression after treatment with IL-1β or infection with microbiota. Wehkamp and collegues showed that the parallel activation of NF-κB and AP-1 is needed for full transcriptional activation of the hBD-2-promotor after IL-1β stimulation, treatment of keratinocytes with supernatants of Pseudomonas aeruginosa , or E. coli mediated hBD-2 induction . On the other hand another study demonstrated induction of hBD-2 expression through Fusobacterium nucleatum in human gingival epithelial cells independent of NF-κB activation  and there is also evidence for induction of hBD-2 through Salmonella enteritidis  or H. pylori  in the absence of functional AP-1. In the present work we were able to show that both NF-κB and AP-1 activation are required for full upregulation of the hBD-2 mRNA after treatment with either supernatants of C. albicans or the coculture of C. albicans with PMNs. Inhibiton of the NF-κB or of the MAPK/AP-1 pathway significantly reduced the induction of hBD-2 expression confirming the central role of both transcription factors. These observations are in line with recent results for H. pylori infection  and the effects of lactobacilli and the VSL#3 bacterial mixture on enterocytes . The role of NF-κB is less clear in the regulation of hBD-3 expression. In contrast to a recently reported role of NF-κB in the regulation of the hBD-3 gene in keratinocytes  the majority of data failed to show a functional relevance for NF-κB in controlling hBD-3 expression [33, 46]. By using siRNA targeting the RelA/p65 subunit of NF-κB we could demonstrate that C. albicans mediates hBD-3 upregulation through a MAPK/AP-1 pathway independently of the observed NF-κB acitvation. To investigate the proposed role of PMNs in the immune response the C. albicans infection [25, 26] we established an in vitro model (Figure 2). PMNs alone induced NF-κB and AP-1 leading to hBD-2 and hBD-3 expression. Coincubation of PMNs with C. albicans lead to a significant upregulation of hBD-2 and hBD-3 expression compared to effects of PMNs or C. albicans alone. Inhibition of the MAPK/AP-1 pathway reduced the expression of both hBDs under this condition. The fact that PMNs alone induced hBD expression indicated that PMNs could contribute to the induction of antimicrobial peptides in epithelia during inflammation. There is some evidence that a TLR-4 mediated interplay between PMNs and epithelial cells is important for the protective response against Candida infections  but the exact mechanisms remained elusive. One the one hand PMNs induced expression and release of IL-6 and IL-8 in O21 cells (Additional file 1) which might in turn activate NF-κB and AP-1 leading to hBD-2 and hBD-3 expression. On the other hand the induction of a TH1 response, i.e. TNF-α and interferon-γ might directly induce or enhance the expression of hBDs as reported recently . On the other hand PMNs are able to establish an anti-fungal response by upregulate the expression of TLR4 in epithelial cells . The observed more pronounced effect on hBD-3 expression are in line with the theory that hBD-3 might be clinically more relevant than hBD-2 since hBD-2 and hBD-3 have potent fungicidal activity against C. albicans at micromolar concentrations, with hBD-3 being about 10 times more fungicidal than hBD-2 [16, 17, 19]. Finally we demonstrated that hBD-3 expression was induced by transactivation of the EGFR independent of EGF (Figure 8 and 9). Many signals besides EGF converge and result in EGFR-dependent signaling which is important for various biological processes including normal growth, development and as shown recently for inflammation or innate immune response [48, 49]. Using blocking antibodies for the EGFR-ligands EGF, HB-EGF, amphiregulin and TGF-α we were able to show that most likely TGF-α is involved in the EGFR mediated upregulation of hBD-3 expression (Figure 9). Since we are also able to inhibit the induction of hBD3 by the supernatants of 1 × 105 C. albicans cells/ml by EGFR and/or TGF-α blocking antibodies we speculate that the EGFR-ligand is secreted by OE21 cells. Ligands of the EGFR are expressed as transmembrane precursors. These are released from the cell surface following shedding of the extracellular domain by a family of metalloproteinases (a disintegrin and metalloprotease (ADAM)). ADAM10, -12 and -17 are the sheddases of the EGFR ligands in response to various stimuli. Since human cathelicidin cationic antimicrobial protein (hCAP)-18 and its active peptide LL-37 have been shown to be involved in the transactivation of the EGFR at the airway epithelial surfaces [50, 51] it is tempting to speculate that the activation of PMNs by C. albicans leads to secretion of proinflammatory mediators including leukotriene B4 (LTB4) and LL-37 [52, 53] which in turn amplifies the inflammatory response [54, 55] and leads to the shedding of EGFR-ligands.
In conclusion we were able to establish a complex in vitro model for Candida infection of esophageal cells to investigate the signalling events leading to upregulation of hBD-2 and hBD-3 expression.
This analysis of signal transduction events in esophageal Candida infection demonstrated a potential functional interaction of epithelial cells with PMNs and that EGFR, NF-κB and MAPK/AP-1 are involved in divergent signalling events governing hBD-2 and hBD-3 expression. Especially the observed effect of PMNs on hBD expression might explain the high incidence of Candida esophagitis and Candida related deaths in neutropenic patients.
Cell culture medium was purchased from PAA-Labs (Linz, Austria), fetal bovine serum from Seromed (Berlin, Germany), recombinant TNF-α from Sigma. Supershift and Westernblot p65, p50 and c-Jun antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA).
Organism and Cell culture
OE21 cells, a moderately differentiated oesophageal squamous carcinoma cell line were cultured in RPMI 1670 supplemented with 10% fetal calf serum and divided every 3 days. Cells for experimental purposes were cultured in 6 well cell culture plates with the same medium and used at subconfluence. C. albicans (a clinical isolate from oesophagitis ) was taken fom a frozen strain suspension (BHI, glycerine). 10 μl was transferred into 10 ml TSB-Bouillon and incubated overnight at 37°C. The cells were then separated from the medium by centrifugation at 2800 × g for 5 min, washed two times with PBS, and finally resuspended in RPMI for each experiment. The final concentration of C. albicans was 1 × 104/ml.
OE21 cells were pre-treated with specific inhibitors or neutralizing antibodies for 30–60 min prior to and during Candida or cytokine stimulation. The neutralzing antibodies against the EGFR (Upstate, USA), TGF-α (R&D Systems), HB-EGF (R&D Systems), amphiregulin (R&D Systems), and EGF (Pepro Tech, Germany) were used at the indicated concentrations. Inhibitors utilized were: PD98059 (Calbiochem) at 10 μM, SB203580 (Tocris, Ellusville, USA) at 10 μM, SP600125 (Tocris, Ellusville, USA) at 20 μM. Inhibitor were disolved in DMSO and DMSO was used as a vehicel control.
Generation of Supernatants from C. albicans
To analyze the effects of secreted factors from C. albicans on OE21 cells, C. albicans were cultured with respective medium for 48 h in the indicated density (0.5 × 104 to 1 × 105 cells/ml). Before use, these conditioned media were precleared by centrifugation (10,000 rpm for 10 min) and used for additional experiments.
Isolation of polymorphonuclear leukocytes (PMN)
PMN were purified using LeucoSep tubes according to the instructions of the manufacturer (Greiner Bio-One). In brief, 3 ml of Ficoll-Paque was preloaded in a 14 ml LeucoSep tube by centrifugation for 30 s at 1,000 g. The heparinized whole-blood samples of healthy volunters were diluted with equal volumes of PBS, and 6 ml of the diluted blood was added to a LeucoSep tube. The cell separation tubes were centrifuged for 15 min at 800 g without braking at room temperature. The cell suspension was collected, and the cells were washed twice in PBS (for 10 min at 640 and 470 g, respectively, for the two successive wash steps) and resuspended in complete RPMI medium before counting.
Stimulation with Supernatants, direct cell contact and transwell coculture model
2 × 105 OE21 cells were seeded into a six-well culture plate. For stimulation experiments medium was replaced after 24 h with the C. albicans supernatants and cultured for indicated times. For direct cell contact suspension cultures of C. albicans (final concentration 0.5 × 104 cells/ml) or PMNs (final concentration 0.5 × 106 cells/ml) were added to the OE21 cells after initial 24 h culture and incubated for the indicated times. In the transwell setting (for a schematic representation please refer to Figure 2) 2 × 105 OE21 cells were cultured into the bottom compartment of a six-well culture plate. After 24 h PMNs or C. albicans were added to the bottom compartment and the corresponding cell type was seeded into the top transwell compartment (Costar GmbH, Bodenheim, Germany) and cultured for additional 24 h.
RNA isolation and cDNA synthesis
Total RNA was isolated using the RNeasy Kit from Qiagen (Hilden, Germany) and reverse-transcribed into single-stranded cDNA, as described previously .
The oligonucleotide sequence and product size for each primer pair used were as described previously .
Quantification of gene expression by real time PCR
Real-time PCR analyses were performed as previously described . Standard curves for each mRNA were constructed by cloning the purified PCR-products containing the target sequence into pCR-Blunt II-TOPO vector (Invitrogen). Concentration of the reference plasmid was measured spectrophotometrically and transformed into number of copies/μl by calculation. The absolute mRNA transcript number in each sample was calculated by use of calibration curves. Identical results were obtained in control experiments.
Culture supernatants were cleared by centrifugation (6.000 rpm, 5 min, 4°C) and protein concentration was determined by the BioRad assays. For a qualitative screening for the cytokine content, samples were adjusted to equal protein concentration and submitted to cytokine antibody arrays (Cytokine Profiler kit, R&D systems) following the manufacturer's instructions.
For knock down of RelA/p65, cells were seeded into 6 well plates (2 × 105 cells/well) and grown overnight, then transfection with 12 μl/well RNAiFect reagent (Invitrogen) and 2 μg/well of either Stealth negative control siRNA (Invitrogen) or Stealth RelA/p65 siRNA (Invitrogen) was performed for 48 h.
Nuclear extracts were prepared as described previously  and incubated with a γ32P-labelled oligonucleotide containing a consensus NF-κB-binding or consensus AP-1 site (Promega; Mannheim, FRG). After 30 min incubation at room temperature, samples were separated by gel electrophoresis at 100 V and 4°C. Gels were dried and exposed to X-ray Hyperfilm (Amersham; Freiburg, FRG).
Data are presented as mean ± SD and analyzed by Student's t-test. A p-value < 0.05 (indicated as * in the figures) was considered as statistically significant.
The authors would like to thank Silvia Voss, Christiane Sandberg, Cornelia Wilgus and Frauke Grohmann for excellent technical support. This work is part of a PhD thesis (N.S.) and a MD thesis (R.P.). This study was supported by grants of the German Research Society (DFG/SFB617) to K.K., P.R. and A.A.
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