Figure 4
From: Conserved cryptic recombination signals in Vκ gene segments are cleaved in small pre-B cells
Vκ cRS cleavage is detected in C57BL/6 bone marrow pre-B cells and 103/BCL2 cells. LM-PCR was conducted with and without ligase on DNA extracted from pre-B cells of C57BL/6 mice or from 103/BCL2 cells after 0 and 3 days at 39°C (panel A) or from 103/BCL2 cells after 0, 1, 3, and/or 5 days at 39°C (panel B). Vκ 6 cRS SE (expected product size: 217 bp and 277 bp) were detected in 103/BCL2 cells and C57BL/6 bone marrow pre-B cells (panel A). LM-PCR product from primary Jκ rearrangements demonstrates that 103/BCL2 cells are rearranging their κ loci (panel B). Vκ cRS SEs were detected in Vκ2, Vκ5, Vκ6, Vκ11, and Vκ17 gene segments in 103/BCL2 cells (panel B). CD14 PCR demonstrates the equivalence of genomic template.