Figure 5

Expression of gallin mRNA in hen oviduct tissue; magnum, isthmus and shell gland measured by RT-QPCR (n = 8, mean ± sem). The expression is corrected for GAPDH expression to attempt to normalise for any differences in tissue sample size and is presented on a logarithmic scale because of the large difference in expression between shell gland and magnum. The different shading for the magnum and isthmus indicate the proportion of expression of the 3 forms of gallin (indicated in the inset key). Note that the proportions of the 3 forms are indicated on the arithmetic scale. Differences between all tissues are significant at P < 0.001.