Table 1 Primers used in this study
From: Peripheral blood T Regulatory cell counts may not predict transplant rejection
Gene | Primer | annealing temperature | |
|---|---|---|---|
TGF-β1 | sense | 5'-CTGCTGCCGCTTCTGCTC-3' | 56°C |
antisense | 5'-TGCGCTTCCGTTTCACC-3' | ||
IL-10 | sense | 5'-CAGACCCACATGCTCCGAGA-3' | 51°C |
antisense | 5'-CAAGGCTTGGCAACCCAAGTA-3' | ||
Foxp3 | sense | 5'-ACCTCCTCTTCTTCCTTGAACC-3' | 56°C |
antisense | 5'-GCATAAAGTGTGGTCTGTCCTG-3' | ||
GAPDH | sense: | 5'-CGGCAAGTTCAACTTCACA-3' | 56°C |
antisense | 5'-AAGACGCCAGTAGACTCCACGA-3' | ||