Figure 5

Specific Reduction of pSTAT3, but not pSTAT5 in Dexamethasone Treated CAIA Mice Overtime Corresponds with Local Dexamethasone Pharmacokinetics. Using our CIA mouse model of RA we treated 35 day mice with either vehicle alone or dexamethasone and looked overtime at the loss of pSTAT3 and pSTAT5 in the paws using our extraction method. (A) Paws from treated mice were processed and analyzed using Luminex^® technology, rapid decrease in pSTAT3 observed within 4 hours post dexamethasone treatment and remains decreased as shown for the 6 hour time point compared to vehicle alone, *p < 0.05. (B) No significant change observed for pSTAT5, trend decrease in overall pSTAT5 levels, ns = not significant as compared to vehicle alone. (C) PK of dexamethasone in the plasma, spleen and paw extracts from treated mice, see materials and methods for complete methods for PK analysis. All graphs show Mean ± SEM, N = 4 mice per group.