Figure 8

Expression of IFN-γ and IL-10 in CD4⁺ and CD8⁺ cells in spleen. Fig. A-C depicts splenocytes at 4 weeks and fig. D-F depicts splenocytes at 8 weeks. (A) Increased expression of IL-10 but not IFN-γ in CD4⁺ cells in high fat diet (HFD) fed mice compared to mice fed chow diet. (B) Increased expression of IL-10 and IFN-γ in CD8⁺ and (C) IFN-γ in CD8⁺CD28⁺ cells in HFD-fed mice compared to chow fed mice. (D) Increased production of IL-10 in CD4⁺ cells but not (E) CD8⁺ cells and no difference in IFN-γ production in (D) CD4⁺, (E) CD8⁺ cells or (F) CD8⁺CD28⁺ cells in high fat diet (HFD) fed mice compared to mice fed chow diet. **P < 0.01, ***P < 0.001.