Table 1 PCR primer sequences and Tm used for the RT-PCR analysis
From: Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cells
Primers | Sequence (5'-3') | Tm |
|---|---|---|
Beta-actin 5' | CAC TAT TTG GCA ACG AGC GG | 60°C |
Beta-actin 3' | TCC ATA CCC AAG AAG GAA GGC | 60°C |
IL-2 5' | TTG AGT GCC AAT TCG ATG ATG | 55°C |
IL-2 3' | AGA TGA TGC TTT GAC AGA AGG CTA | 55°C |
IL-4 5' | CGG AGA TGG ATG TGC CAA AA | 60°C |
IL-4 3' | GCA CCT TGG AAG CCC TAC AG | 60°C |
IFN-γ 5' | CCT GGG GCC TAG CTC TGA | 60°C |
IFN-γ 3' | CAG CCA GGA ACA GCC ATG AG | 60°C |
GATA3 5' | CGA GAT GGT ACC GGG CAC TA | 60°C |
GATA3 3' | GAC AGT TCG CGC AGG ATG | 60°C |
Tbet 5' | TTC CCA TTC CTG TCC TTC ACC | 60°C |
Tbet 3' | TGC CTT CTG CCT TTC CAC AC | 60°C |