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Figure 1 | BMC Immunology

Figure 1

From: Influence of variable domain glycosylation on anti-neutrophil cytoplasmic autoantibodies and anti-glomerular basement membrane autoantibodies

Figure 1

Identification of the binding specificity of SNA. A: Western-blot analysis of intact IgG, IgG-Fab and IgG-Fc after papain digestion. a: Detection with mouse polyclonal anti-Fab antibodies. Lane 1: intact IgG; lane 2: flow-through fraction of protein A-Sepharose; lane 3: eluted fraction of protein A-Sepharose. b: Detection with mouse polyclonal anti-Fc antibodies. Lane 4: intact IgG; lane 5: flow-through fraction of protein A-Sepharose; lane 6: eluted fraction of protein A-Sepharose. c: Detection with SNA. Lane 7: intact IgG; lane 8: flow-through fraction of protein A-Sepharose; lane 9: eluted fraction of protein A-Sepharose. B: Comparison of variable region glycosylation levels of plasma total IgG between patients and normal controls. Normal: 20 normal controls; group 1: 10 patients with anti-MPO antibodies; group 2: 6 patients with both anti-GBM antibodies and anti-MPO antibodies; group 3: 6 patients with anti-GBM antibodies without anti-MPO antibodies; group 4: 5 patients with anti-PR3 antibodies.

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BMC Immunology

ISSN: 1471-2172

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