Understanding mechanisms of vitiligo development in Smyth line of chickens by transcriptomic microarray analysis of evolving autoimmune lesions

Table 3 Relative expression of DE genes in SL samples^a from microarray vs. qRT-PCR analysis^b

^a, SL samples included NV, EV, AV and CV samples which were from SL chickens that never developed vitiligo; from vitiliginous SL chickens within 1 week before SLV onset, during active depigmentation and at least one week after complete loss of pigmentation, respectively
^b, Gene expression in NV, EV, AV and CV was presented by fold changes relative to expression in BL samples in microarray and in qRT-PCR, where fold-change was determined by the delta delta Ct method. The same RNA pools for NV, EV, AV, CV and BL samples used in the microarray study were reverse transcribed to cDNA, which was subjected to qPCR with GAPDH as the endogenous control gene and cDNA from BL sample as the calibrator. All values are fold change means of three replications analyzed by JMP Genomics 4 for microarray and SYSTAT for qRT-PCR data

ISSN: 1471-2172