Figure 5

(A) The effects of GMFG knockdown on CCRF-CEM T cell integrin expression levels and functional activities. A) The levels of β1, α4 and α5 integrin expression were determined by flow cytometry for a non-silencing control and GMFG knockdown cells (representative example of 3 experiments). B) The effects of a control (HSA), or adhesion blocking antibodies to integrin on the adhesion of control and GMFG knockdown cells to fibronectin. C) The effects of an RGD containing peptide on the adhesion of non-silencing and GMFG knockdown cells to fibronectin. In the absence of peptide, the number of adherent GMFG knockdown cells was 91% higher than control cells ( p < 0.001). The RGD peptide did not have any significant effect on the adherence of non-silencing control cells. Data is shown for cell lines created from CCRF-CEM T cells and is representative of two separate experiments. D) The effects of GMFG knockdown on the expression levels of the high-affinity binding conformation of β1 integrin. The control and GMFG-KD cells were stained with HUTS-1. HUTS-1 staining was greater in knockdown cells (grey and dotted lines) than in comparable non-silencing control cells (black line). Data is representative of two separate experiments.