Figure 3
In vivo survival, infection ability and construct stability of six single-recombinant Salmonella -EGFP strains. (A) Survival ability and construct stability of the six single-recombinant Salmonella -EGFP strains in mice feces. A (1) CFU results for the six single-recombinant strains in mice feces, on days 1, 3 and 7 after oral inoculation. Each bar in the graph represents the mean CFU/g of mice fecal samples randomly collected from six mice in each group. Error bars show the standard deviation. ** indicates that the mean CFU/g was significantly different from those of other strains ( P < 0.01). The SL7207 strain was treated identically as a control. A (2) Construct retention rates for the six single-recombinant strains in mice feces on days 1, 3 and 7 after inoculation. ** indicates that the mean of the construct retention rate in this group was significantly different from those of the other strains ( P < 0.01). (B) and (C) show the infection ability and construct stability results for the six single-recombinant Salmonella -EGFP strains in mice spleen (B) and mesenteric lymph nodes (C). B (1) and C (1) show the CFU results in mice spleen and mesenteric lymph nodes on days 1, 3 and 7 after inoculation, respectively. Each ● represents one mouse. Red lines indicate the average CFU/g for each strain. The SL7207 strain was used as a control. B (2) and C (2) show the construct retention rates for the six single-recombinant strains in mice spleen and mesenteric lymph nodes on days 1, 3 and 7 after inoculation, respectively. In A (2), B (2) and C (2), each percentage is the mean construct detection rate based on six mice samples in each group. N/A represents the construct detection rate could not be accurately calculated at this time point.