Table 1 Plasmids and strains used in this study

p Bluescript II SK

Amp^R; Cloning vector, p UC origin 500–700 copy per cell

p MD18-T

Amp^R; Cloning vector, p UC origin 500–700 copy per cell

p Sim6

Amp^R,Lambda-red recombinase plasmid

p loxp-cm-loxp

Amp^R, Cm^R ; p BSK derivative containing EGFP and loxp-cm-loxp fragment

p EGFP

Km^R ; CMV promoter drived EGFP expression, p UC origin 500–700 copy per cell

p ACYC177

Cm^R ; p 15a origin plasmid, 10–12 copy per cell

p VAX-1

Km^R ; CMV promoter; p UC origin 500–700 copy per cell

p HP

Amp^R; p BSK derivative containing prosseA promoter, egfp gene

p HAOP

Amp^R; p BSK derivative containing prosseA promoter, ha(91–261) gene

p CP-cm

Amp^R, Cm^R ; p BSK derivative containing prosseA promoter, egfp gene, and loxp-cm-loxp fragment

p LP

Cm^R ; p ACYC177 derivative containing prosseA promoter, egfp gene

p OmpA

Amp^R; p MD18-T derivative containing lac promoter, and lpp-ompA gene

p HO

Amp^R; p MD18-T derivative containing lac promoter, and lpp-ompA-egfp gene

p O-HAOP

Amp^R; p MD18-T derivative containing lac promoter, and lpp-ompA-ha(91–261) gene

p CO-cm

Amp^R, Cm^R ; p BSK derivative containing prosseA promoter, lpp-ompA-egfp gene, and loxp-cm-loxp fragment

Strains

S. typhimurium

SL7207

his G46 DEL407 [aroA::Tn10 {Tc^s}];

HP

Amp^R; SL7207; with plasmid p HP

LP

Cm^R ; SL7207; with plasmid p LP

CP

Cm^R ; SL7207; htrA<>prosseA-egfp-cm

HO

Amp^R; SL7207; with plasmid p HO

CO

Cm^R ; SL7207; htrA<>prosseA-lpp-ompA-egfp -cm

E

Km^R ; SL7207; with plasmid p EGFP

CP + HP

Cm^R ; Amp^R; SL7207; CP with plasmid p HP

CP + LP

Cm^R ; SL7207; CP with plasmid p LP

CP + HO

Cm^R ; Amp^R; SL7207; CP with plasmid p HO

CP + E

Cm^R ; Km^R ; SL7207; CP with plasmid p EGFP

E + LP

Cm^R ; Km^R ; SL7207; LP with plasmid p EGFP

C-HAOP

Amp^R; SL7207; with plasmid p HAOP

O-HAOP

Amp^R; SL7207; with plasmid p O-HAOP

BSK

Amp^R; SL7207; with plasmid p BSK

ACYC177

Cm^R ; SL7207; with plasmid p ACYC177

OmpA

Amp^R; SL7207; with plasmid p OmpA

VAX-1

Km^R ; SL7207; with plasmid p VAX-1

VAX-1 + ACYC177

Cm^R ; Km^R ; SL7207; with plasmid p VAX-1 and p ACYC177

E. coli

DH10B

endA1 recA1 galE15 galK16 nupG rpsL ΔlacX4 Φ Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-