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Figure 1 | BMC Immunology

Figure 1

From: Boosting immune response with the invariant chain segments via association with non-peptide binding region of major histocompatibility complex class II molecules

Figure 1

Constructed, expressed and purifiedhybrids containing Ii-segments andantigen peptides. A. Schematic diagram of hybrids containing Ii-segments and antigen peptides. a. Full-length Ii consists of cytosolic domain (Cyt), transmembrane domain (TM) and luminal domain, which includes an Ii-key sequence ( L1), CLIP ( L2), DN sequence ( L3) and trimerization region ( L4). b. Components of reconstructed hybrids. These hybrids contain different Ii-segments and a multiepitope, F306. c. Structure of F306. F306 consisted of three potential epitopes in fusion protein of Newcastle disease virus and had a predicted molecular weight of 11.2 kDa. B. PCR-amplified gene and hybrid DNA segment products: mouse H2-Aa , H2-Ab , full-length Ii , Ii /F306, multi-epitope F306 and the hybrids, Ii-key/F306, Ii-key/F306/DN, Cyt/TM/Ii-Key/F306/ and Cyt/TM/Ii-Key/F306/DN. C. Expressed and purified products. PCR-amplified F306 and hybrid DNA were cloned into prokaryotic expression vectors, pGEX-4 T-1 or pET-32a, and expressed. Purified products: GST-Ii/F306, GST-F306, GST-Ii-key/F306, GST-Ii-key/F306/DN, GST-Cyt/TM/Ii-Key/F306, and GST-Cyt/TM/Ii-Key/F306/DN for immunization and His-F306 for coating in ELISA.

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BMC Immunology

ISSN: 1471-2172

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