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Figure 2 | BMC Immunology

Figure 2

From: Boosting immune response with the invariant chain segments via association with non-peptide binding region of major histocompatibility complex class II molecules

Figure 2

Binding of Ii, Ii-segments,Ii-segments/F306 or Ii/F306 hybridsto MHC II β chain. Mouse H2-Ab/Myc was cotransfected with mouse Ii gene Ii/GFP (1), Ii /F306 hybrids (2), Cyt/TM/Ii-key/F306/DN/GFP (3), Cyt/Ii-key/F306/DN/GFP (4) or Ii-key/F306/DN/GFP (5) in COS7 cells. After 36 h, the cells were lysed and the antibody against Myc was added for immunoprecipitation. Subsequently, the immune complexes (lanes 1–5), cell lysates transfected with only H2-Ab/Myc (lane 6) and control cells without transfection (lane 7) were separated by SDS-PAGE, immunoblotted and detected with antibodies against GFP (WB1) or Myc (WB2). These fusion proteins had molecular weights of 51.0 kDa (Ii/GFP), 58.9 kDa (Ii/F306/GFP), 45.7 kDa (Cyt/TM/Ii-key/F306/DN/GFP), 31 kDa (Cyt/Ii-key/F306/DN/GFP) and 27.7 kDa (Ii-key/F306/DN/GFP), in which GFP had a molecular weight of 27 kDa.

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BMC Immunology

ISSN: 1471-2172

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