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Figure 3 | BMC Immunology

Figure 3

From: Boosting immune response with the invariant chain segments via association with non-peptide binding region of major histocompatibility complex class II molecules

Figure 3

Interaction between MHC class II molecule and Ii-segments or hybrids in co-transfected COS7 cells. Cells were transiently co-transfected with the reconstructed pEGFP-N1-H2-Aa or pEGFP-N1-H2-Ab expressing gene of fusion protein (GFP/MHC class II alpha or beta chain) and the reconstructed pmCherry-C1s expressing genes of fusion protein (RFP/Ii hybrids or RFP/Ii-segments), respectively. After 24 h, the cells were observed using CLSM and × 60 oil objective. a) Mouse Ii and MHC II alpha or beta chain. b) Mouse MHC II alpha or beta chain and Ii/F306 hybrid. Both molecules co-localized with MHC II molecules to plasma membrane visualized in the merged images represented in orange. c) Mouse MHC II alpha or beta chain and Cyt/Ii-key/CLIP/DN. d) Mouse MHC II alpha or beta chain and Cyt/Ii-key/F306/DN. Both Ii-segments localized in cytosol or to plasma membrane and appeared with absent co-localization with MHC II molecules as visualized in the merged image. e) Mouse MHC II alpha or beta chain and Cyt/TM/Ii-key/CLIP/DN hybrids. f) Mouse MHC II alpha or beta chain and hybrids Cyt/TM/Ii-key/F306/DN. Both hybrids localized on plasma membrane, but appeared with relative weak interaction with MHC II molecules visualized in the merged image represented in dispersive yellow-orange, respectively.

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BMC Immunology

ISSN: 1471-2172

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