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Table 1 Primers, cloned Ii-segments andreconstructed vectors in this study

From: Boosting immune response with the invariant chain segments via association with non-peptide binding region of major histocompatibility complex class II molecules

No.

 

Primer sequences (5´-´)

Reconstructed vectors

1

Forward:

5'gaagatctaatgccgtgcagcagagc3'

pEGFP-N1-H2-Aa (1)

 

Reverse:

5'gcgtcgacactaaaggccctggg3'

 

2

Forward:

5'gaagatcttatggctctgcagatcccc3'

pEGFP-N1-H2-Ab (1)

 

Reverse:

5'gcgtcgacatcgcaggagccct3'

 

3

Forward:

5'cccgaattcacatggctctgcagatccc3'

pCMV-Myc-H2-Ab (2)

 

Reverse:

5'ggaagatctatcactgcaggagccct3'

 

4

Forward:

5'cccgaattctatggctctgcagatcccc3'

pmCherry-C1-Ii (1)

 

Reverse:

5'gcgtcgactcacagggtgacttga3'

pEGFP-C1-Ii (3)

5

Forward1:

5'ccgctcgagacatgcaacgcgacct3'

pmCherry-C1-Ii/F306 (1)

 

Reverse1:

5'atgagcaagaactccctggaggagaag

pEGFP-C1-Ii/F306 (3)

 

Forward2:

aagcccacagagg3'

 
 

Reverse2:

5'ctcctccagggagttcttgctcatctcaaa

 
  

caagagccactgc3'

 
  

5'cgggatcctcacagggtgacttgaccc3'

 

6

Forward:

5'gaattcgatgcttcgcatgaagcttcc3'

pmCherry-C1-Ii-Key/CLIP/DN (1)

 

Reverse:

5'gtcgacctagttatccatgaacat3'

 

7

Forward:

5'ccggaattcgatgcaacgc3'

pmCherry-C1-Cyt/Ii-Key/CLIP/DN (1)

 

Reverse:

5'gtcgacctagttatccatggacat3'

 

8

Forward:

5'gaattcgatgcttcgcatgaagcttcc3'

pmCherry-C1-Cyt/TM/Ii-Key/CLIP/DN (1)

 

Reverse:

5'gtcgacctagttatccatggacat3'

 

9

Forward:

5'ccgctcgagacatgcttcgcatgaag3'

pmCherry-C1-Ii-key/F306/DN (1)

 

Reverse:

5'cgggatcctcagttatcataaatacc3'

pEGFP-C1-Ii-key/F306/DN 3)

10

Forward:

5'ccggaattcgatgcaacgc3'

pmCherry-C1-Cyt/Ii-Key/F306/DN (1)

 

Reverse:

5'ccgctcgagctagttatccat3'

pEGFP-C1-Cyt/Ii-Key/F306/DN 3)

11

Forward:

5'ccgctcgagatatgcaacgcgacctca3'

pmCherry-C1-Cyt/TM/Ii-Key/F306/DN (1)

 

Reverse:

5'cggcatcctcagttatcataaatacc3'

 

12

Forward:

5'ccggaattcatgctcccaaatatg3'

pEGFP-C1-Cyt/TM/Ii-Key/F306/DN (3)

 

Reverse:

5'ccgctcgagtcaataaataccagg3'

 

13

Forward:

5'ccggaattcatgcttcgcatgaagctc3'

PGEX-4T-1-Ii-key/F306 (4)

 

Reverse:

5'ccgctcgagtcaataaataccaggag3'

 

14

Forward:

5'ccggaattcatgcttcgcatgaagctc3'

PGEX-4T-1-Ii-key/F306/DN (4)

 

Reverse:

5'ccgctcgagtcaataaataccagg3'

 

15

Forward:

5'cgggatccatgcaacgcgacct3'

PGEX-4T-1-Cyt/TM/Ii-Key/F306 (4)

 

Reverse:

5'ccgctcgagtcaataaataccag3'

 

16

Forward:

5'cgggatccatgcaacgcgacct3'

PGEX-4T-1-Cyt/TM/Ii-Key/F306/DN (4)

 

Reverse

5'ccgctcgagtcagttatcaccagga3'

 

17

Forward:

5'cgggatccatgcaacgcgacct3'

PGEX-4T-1-Ii/F306 (4)

 

Reverse:

5'cgggatcctcacagggtgacttgaccc3'

 

18

Forward:

5'ccggaattcatgctcccaaatatgcctaag3'

PGEX-4T-1-F306 (4)

 

Reverse:

5'ccgctcgagtcaataaataccaggagacataggg3'

pET-32a-F306 (5)

  1. The design of all the primers was based on the reported cDNA sequences: mouse Ii from GenBank ID: NM_010545, mouse H2-Aa from GenBank ID: NM_010378, mouse H2-Ab from GenBank ID: AY_452202 and NDV F gene from GenBank ID: AY_508514. The reconstructed vectors were used for CLSM (1), for IP (2), for WB (3), for expression as immunization antigens (4)or for expression as coating antigen in ELISA (5).
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BMC Immunology

ISSN: 1471-2172

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