New perspectives for natural antimicrobial peptides: application as antinflammatory drugs in a murine model

BMC Immunology

Table 6 NO ₂ ^- production of J774 cells: Mouse macrophages untreated, treated with RJI-C, TB-KK or the MIX, stimulated with LPS, stimulated with LPS and treated with RJI-C, TB-KK or the MIX

Treatment	Time of incubation (h)
Treatment	24	48	72
No treatment	0.25 ± 0.04	0.69 ± 0.02	0.92 ± 0.2
RJI-C (15 μg/ml) (12 μM)	0.42 ± 0.03	0.75 ± 0.01	1.02 ± 0.3
TB-KK (15 μg/ml) (6.5 μM)	0.82 ± 0.05	1.25 ± 0.2	1.34 ± 0.2
MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) (RJI-C 7.3 μM + TB-KK 2.6 μM)	0.72 ± 0.3	0.85 ± 0.3	1.06 ± 0.2
LPS (10 μg/ml)	2.93 ± 0.2	10.96 ± 0.4	12.16 ± 0.5
LPS + RJI-C (15 μg/ml) (12 μM)	2.85 ± 0.3	8.42 ± 0.1	10.21 ± 0.2
LPS + TB-KK (15 μg/ml) (6.5 μM)	3.12 ± 0.6	9.75 ± 0.1	11.45 ± 0.2
LPS + MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) (RJI-C 7.3 μM + TB-KK 2.6 μM)	2.63 ± 0.4	7.25 ± 0.3	8.26 ± 0.1

Data are expressed as micromoles of NO2- for 106 input cells, and are means ± standard deviation of three different experiments each performed in triplicate.

ISSN: 1471-2172