Figure 2

Changes of CD3 and CD5 levels following adult thymectomy. A) Spleen cells from control and adult thymectomized mice were stained with anti-CD4-PE, anti-CD3-FITC and anti-CD5-APC, or anti-CD8-PE, anti-CD3-FITC and anti-CD5-APC antibodies and analyzed by flow cytometry. Shown are ratios of CD5 mean fluorescence intensities obtained on gated CD4⁺ or CD8⁺ spleen cells from matched control and thymectomized individual mice (four mice per group each time point). B) Spleen cells from the same set of mice were stained with anti-CD4-PE, anti-CD62L-FITC and anti-CD44-APC antibodies and analyzed by flow cytometry. Shown are percent CD62L^hi or CD44^hi cells on gated CD4⁺ spleen cells. C) Shown are ratios of CD3 mean fluorescence intensities obtained on gated CD4⁺ or CD8⁺ spleen cells from matched control and thymectomized individual mice, stained as described in (A)