Figure 3
Administration of ATRA suppressed the levels of Th2- and Th17-related cytokines in draining lymph nodes. A. Mice were sacrificed at 24 hours after the final challenge and lymphocytes were isolated from mediastinal lymph nodes and stimulated with OVA323–339 peptide for 72 hours. The percentages of IL-4 positive and IFN-γ positive CD4 T cells were analyzed by FACS. The results showed that ATRA pretreatment decreased the percentages of IL-4 positive T cells compared with the vehicle mice. B. The concentration of IL-4, IL-5, IL-10, and IL-17A in supernatants of cultured mediastinal lymph nodes were detected by ELISA. The levels of IL-4, IL-5, and IL-17A were significantly decreased after pretreatment with ATRA. There was no significant difference in IFN-γ and IL-10 among the three groups. Data represent the mean ± SEM with n = 10 for each group (***, p < 0.001). Data are representative of 3 independent experiments.