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Figure 2 | BMC Immunology

Figure 2

From: CTLA4-Ig (abatacept) therapy modulates T cell effector functions in autoantibody-positive rheumatoid arthritis patients

Figure 2

Flow cytometry staining for intracellular cytokines TNF, IFN-γ, IL-17A. PBMCs were stimulated in vitro with α-CD3 for 6 h or 5 days and intracellular cytokine staining was performed for IFN-γ, TNF and IL-17A. The plotted cells are gated as CD14-CD3+CD4+CD28+ T cells. (A) A representative FACS staining. Left row: Unstimulated cells. Subsequent rows: α-CD3 stimulation at baseline and 6 months following treatment. (B-E) Intracellular cytokine stainings. (B) ACPA-positive patients, 6h stimulation (n=12). (C) ACPA-negative patients, 6h stimulation (n=7). (D) ACPA-positive patients, 5days stimulation (n=12). (E) ACPA-negative patients, 5days stimulation (n=6).

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BMC Immunology

ISSN: 1471-2172

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