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Figure 4 | BMC Immunology

Figure 4

From: Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors

Figure 4

Hoxa9 is not required for DC differentiation. A) Flow cytometric comparison of DC subsets in BM of wildtype B6, flt3l−/−, and hoxa9−/− mice. BM cells were first analyzed for MHCII expression. The MHCII + cells were then fractionated based on differential expression of CD45R/B220 into MHCII+B220+ or MHCII+B220-. Conventional DCs are MHCII+B220-CD11c+. Plasmacytoid DCs are MHCII+B220+ CD11c+. B) pDC (white bars) and cDC (gray bars) precursor frequency in BM. C) Absolute numbers of pDC (white bars) and cDC (gray bars) in BM. Precursor frequencies were calculated by multiplying sequential percentages of each gated region as described in Figure1. Absolute numbers were determined by multiplying precursor frequencies x numbers of BM mononuclear cells obtained from 4 hind limb leg bones. Data is representative of 4 mice per genotype. Error bars represent SEM.

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BMC Immunology

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