Figure 2

Effects of inhibitors on Streptococcus sanguinis induced sCD40L (A), VEGF (B), SDF-1 (C) and MMP-1 (D) secretion from platelets. Platelets were pre-incubated with either aspirin (100 μM; 20 mins.; n = 7), wortmannin (100 nM; 2 mins; n = 5), RGDS (1 mM; 5 mins; n = 5), AN51 (0.165 mg/mL, 5 mins; n = 5) or the saline control then stirred with S. sanguinis strain 2017–78. Release reactions were terminated and the levels of sCD40L in the releasates determined by multi-plex luminex. Results are shown as box plots with whiskers for the data range representing median, upper and lower quartiles of several individual experiments using the platelets from different donors and subsequently analyzed using the Mann–Whitney U-test (*P < 0.05 with respect to saline control; ^#P < 0.05 with respect to S. sanguinis strain 2017–78).