Figure 2From: Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligandEffect of combined signals on cytokine gene expression in DC. DC (1 × 106) were treated with CD40L+IL-1β, CD40L+LPS, LPS+IL-1β, LPS+CD40L+IL-1β, CD40L+IFN-γ or LPS+IFN-γ for 8 hours. Following stimulation, RNA was isolated and subjected to RPA analysis (as described in Figure 1). Controls are shown of yeast RNA incubated with probes followed by RNase treatment (background) or mock treatment (undigested probes).Back to article page