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Figure 1 | BMC Immunology

Figure 1

From: Severe anaphylactic reactions to glutamic acid decarboxylase (GAD) self peptides in NOD mice that spontaneously develop autoimmune type 1 diabetes mellitus

Figure 1

Challenge with G7 or PD GAD65 peptides caused anaphylactic shock. NOD mice were not injected ("none") or received 3 weekly i.p. injections with saline (as a control) or the indicated peptide preparations in IFA, and, except for the None/None group, were challenged with saline or peptides in saline i.p. 4 weeks after the last immunization/injection (see Methods). IgE-mediated passive systemic anaphylaxis was induced in mice that had been sensitized with an i.p. injection of anti-DNP IgE and then challenged i.v. 24 h later with DNP-HSA. Body temperature was measured at 5 minute intervals after challenge for 30 minutes or until death. Data are shown as mean +/- s. e. m. (A) Data for all mice, including those that gave no detectable anaphylactic response (i.e., temperature change of <1C° and no clinical signs of anaphylaxis). *, **, **** P < 0.05, 0.01, or 0.0001 vs any negative control group (i.e., None/None, Saline-IFA/Saline, None/G7 peptides) by ANOVA. (B) Data for mice that exhibited anaphylaxis. ***, ****, n.s., P < 0.001, 0.0001, or not significant (P > 0.05) vs G7 Peptides/G7 Peptides.

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BMC Immunology

ISSN: 1471-2172

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