Figure 6

Role of IL-6 in the decreased neutrophil recruitment of p50-deficient mice during LPS-induced pulmonary inflammation. (A) Increased IL-6 in the lungs of p50-deficient mice. E. coli LPS was instilled intratracheally to WT and p50-deficient mice, bronchoalveolar lavage fluids were collected, and IL-6 concentrations were measured by ELISA. Asterisk (*) denotes significant difference from WT mice. (B) Effect of p50 deficiency on LPS-induced neutrophil recruitment in the absence of IL-6. IL-6-deficient and p50-deficient mice were crossed to generate double mutant mice deficient in both IL-6 and p50. E. coli LPS was instilled intratracheally to IL-6-deficient mice and double mutant mice deficient in both IL-6 and p50, and lungs were collected after 24 hours. Emigrated neutrophils in the alveolar air spaces were quantified using morphometric analyses of histologic sections. Asterisk (*) denotes significant differences from IL-6 deficient mice without mutation in the gene for p50.