Gene name | [primer]a (μM) | [Mg++]b (mM) | Annealing conditionsc (°C…s) | Product TMd (°C) | Amplicon Lengthe (bp) |
---|---|---|---|---|---|
βactin | 0.3 | 3.5 | 58°C…4s | 83.5 | 97 |
CypA | 0.4 | 4 | 58°C…5s | 85.2 | 127 |
GAPDH | 0.4 | 3 | 58°C…4s | 86.9 | 202 |
HPRT | 0.4 | 4 | 60°C…5s | 84.7 | 123 |
IL1a | 0.4 | 4 | 65°C…5s | 81.5 | 85 |
IL1b | 0.4 | 3.5 | 58°C…5s | 82.0 | 79 |
IL1RA | 0.4 | 3 | 65°C…5s | 80.7 | 117 |
IL1R1 | 0.4 | 3 | 64°C…5s | 80.0 | 105 |
IL1R2 | 0.4 | 4 | 64°C…5s | 84.5 | 116 |
IL6 | 0.4 | 3 | 65°C…5s | 80.9 | 79 |
IL6R | 0.4 | 5 | 60°C…5s | 84.3 | 117 |
TNFa | 0.4 | 3 | 58°C…4s | 84.7 | 111 |
TNFR2 | 0.4 | 4 | 60°C…5s | 84.9* | 83 |
SOCS3 | 0.4 | 4 | 65°C…5s | 84.7 | 99 |
C/EBPβ | 0.4 | 4 | 59°C…5s | 90.2 | 90.2 |
cFos | 0.4 | 4 | 60°C…5s | 84.6 | 84.6 |
cJun | 0.4 | 4 | 62°C…5s | 89.2 | 89.2 |
COX-2 | 0.4 | 4 | 60°C…4s | 83.8 | 83.8 |
NOS-2 | 0.4 | 4 | 58°C…5s | 83.8 | 83.8 |