Figure 1From: Expression and functional effects of Eph receptor tyrosine kinase A family members on Langerhans like dendritic cellsThe EphA2 receptor tyrosine kinase is expressed by in vitro generated LLDC Adherent mononuclear cells were isolated and cultured one week in the presence of GM-CSF and IL-4 (for monocyte derived dendritic cells, DC) and TGF-β (for monocyte derived LLDC). (A) Cells were incubated with a monoclonal EphA2 antibody (bold line) or an irrelevant antibody (dotted line) followed by staining with PE-labeled α-mouse antibody. (B) Cells were incubated with a soluble EphA2 ligand fusion protein (ephrin-A4-Fc, bold line) and a negative control fusion protein (CD19-Fc, dotted line). (C) A Western blot with cell lysates from monocyte derived dendritic cells (DC) or LLDC (LLDC) were immunoblotted using a polyclonal anti-EphA2 antiserum.Back to article page