Figure 1

Allergen and particle dose-response experiments in BALB/cA and NIH mice Levels of serum OVA-specific IgE (A, D), IgG1 (B, E) and IgG2a (C, F) on day 26 after injection into one hind footpad of 100 (open columns), 50 (gray) or 10 (black) μg OVA combined with 100, 40, 10 or 0 μg PSP, or buffer (HBSS (hatched)), in BALB/cA (A, B, C) and NIH (D, E, F) mice. On day 21 the mice were boostered with the same OVA dose in the footpad. The PSP-control ("PSP ctr") group was given 100 μg PSP on day 0, followed by HBSS injection on day 21 (diamonds). Values (arbitrary units, AU) for individual mice (circles) and median values (columns) for groups of eight mice are shown. Dotted lines indicate the lower or higher detection limits for the ELISA assays. Note different scales for the two strains of mice.