Figure 2

The adjuvant effect of PSP on OVA-specific IgE and IgG2a responses in different strains of mice Levels of serum OVA-specific IgE (A) and IgG2a (B) on day 26 after injection into one hind footpad of HBSS (open columns), 10 μg OVA (gray), 40 μg PSP (hatched) or 10 μg OVA + 40 μg PSP (black) in different mouse strains. On day 21 all mice were boostered with 10 μg OVA in the footpad. Vertical straight lines indicate separate experiments. Values (arbitrary units, AU) for individual mice (circles) and median values (columns) for groups of mice are shown. Dotted lines indicate the lower detection limits for the ELISA assays. Brackets indicate statistically significant differences between groups (p < 0.05). All experiments were performed twice with similar results, with eight mice per group.