Figure 1

ITK regulates proliferation in vitro in response to SEB. Splenocytes and lymph node cells from WT (filled circles) and ITK deficient (open circles) mice were stimulated in vitro with the indicated concentrations of SEB, and proliferative response determined. Time course of proliferation over the indicated doses of SEB a) 5 μg/ml SEB, b) dose curve of 0.0005, 0.005, 0.05, 0.5, & 5 μg/ml SEB analyzed at 5 days post stimulation. Purified WT (filled circles) and ITK deficient (open circles) T cells were stimulated in vitro over the indicated time with c) 10 μg/ml SEB, or a dose curve of 0.01, 0.1, 1, and 10 μg/ml SEB and analyzed at d) 3 days, e) 4 days or f) 5 days post stimulation. * indicates statistically significant difference with a p < 0.05.