Figure 6

ITK is required for SEB mediated induction of c-jun phosphorylation in responding T cells in vivo. WT (top panels) or ITK null (bottom panels) mice were injected with 50 μg SEB or PBS i.v. and sacrificed after 1 hr. Splenocytes were then analyzed for the presence of phosphorylated c-jun in the Vβ8⁺ and Vβ6⁺ T cell populations. a) Vβ6⁺ T cells from WT mice; b) Vβ8⁺ T cells from WT mice; c) Vβ6⁺ T cells from ITK null mice; d) Vβ8⁺ T cells from ITK null mice.