Figure 7

ITK is not necessary for SEB induced upregulation of CD69 inresponding T cells in vivo. WT (top panels) or ITK null (bottom panels) mice were injected with 50 μg SEB or PBS i.v. and sacrificed after 2 hrs. Splenocytes were then analyzed for the presence of CD69 on the Vβ8⁺ and Vβ6⁺ T cell populations. a) Vβ6⁺ T cells from WT mice; b) Vβ8⁺ T cells from WT mice; c) Vβ6⁺ T cells from ITK null mice; d) Vβ8⁺ T cells from ITK null mice.