Figure 3

Binding of GATA factors to CCR3 exon 1. In (A), EMSA assay with probes E1-B (left panel) and E1-C (right panel) and nuclear extracts from AML14.3D10 cells is shown. Three cold competitors were used in each case: the probe itself (E1-B-WT and E1-C-WT, respectively), the probe with the GATA site mutated (E1-B-mut and E1-C-mut, respectively) and the GATA consensus oligonucleotide (GATA cons). A representative experiment, of three similar experiments, is shown. In (B), EMSA assay with the full-length probe (E1-FL) and extracts from eosinophilic AML14.3D10 cells in the presence or absence of antibodies against GATA-1 is shown. The arrow depicts the specific band. In (C), EMSA assay with probes E1-B (left panel) and E1-C (right panel) and nuclear extracts from AML14.3D10 cells and cold competitor probe itself (CC: E1-B and CC: E1-C, respectively) or anti-GATA-1 antibody (GATA-1 atb) is shown.