Figure 2

Real-time PCR analysis of relative mRNA expression levels of Wnt pathway molecules in BM B progenitor sub-populations. The sub-populations ELP, pro-B, large pre-B, small pre-B and immature B (imm.B) were isolated by FACS sorting. The relative mRNA expression levels of Wnt receptors and co-receptors, β-catenin, plakoglobin, LEF-1 and TCF-4 were quantified by real-time PCR analysis. Calculations of the expression levels were performed using the standard curve method and then normalized to the expression of PGK1 mRNA. mRNA levels in pro-B cells were used as calibrators. The bars represent the mean of 3–5 experiments ± SEM.