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Figure 6 | BMC Immunology

Figure 6

From: Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7

Figure 6

Nuclear translocation of TSAd depends on aa239–334. The SH2D2A-5 variant is excluded from the cell nucleus in Jurkat TAg cells: Confocal immunocytochemistry analysis of Jurkat TAg cells transiently transfected with pEF-HA-SH2D2A-2 and -5. The nuclear membrane protein LAP-2 was stained red (Cy™-3), whereas the HA-tagged recombinant proteins are stained green (Cy-2™). Cells were stimulated with anti-CD3 mAbs (OKT3) for 30 min. Horizontal sections are labelled Y, vertical sections are labelled Z (100× objective, 4× zoom).

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BMC Immunology

ISSN: 1471-2172

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