Figure 2
From: Enumeration of leukocyte infiltration in solid tumors by confocal laser scanning microscopy
Effect of threshold value on cell count. Ten cells were selected from a CD3 image stack whose volumes were completely within the 20 optical slices scanned. The total tumor volume occupied by CD3 antigen was calculated at ten threshold values (1–10). The seeded volume of each of the ten selected cells was also calculated at the same threshold values. The total number of cells in the image stack was then determined by dividing the mean cell volume (of the ten cells) into the total CD3 volume at each threshold. Results are expressed as number of cells (+/- SD) at each threshold level. Cell counts remained relatively constant at lower thresholds, but gradually rose at higher thresholds.