Skip to main content
Figure 1 | BMC Immunology

Figure 1

From: Targeted in vitro and in vivo gene transfer into T Lymphocytes: potential of direct inhibition of allo-immune activation

Figure 1

Construction of CD3-p21 chimeric plasmid conctruct. A complete vector structure of pSK-CD3-p21 chimeric plasmid is shown. The restriction enzyme sites for CD3 promoter, enhancer and p21 gene are also shown. Plasmid TGE-CD3 contains the murine T cell-specific delta enhancer and promoter. CD3 promoter was cut with Pst I and blunted with T4 DNA polymerase. HindIII linkers were ligated to the fragment and, following HindIII digestion, cloned into the HindIII site downstream of the enhancer region. The p21 gene was excised with restriction enzymes KpnI and XbaII, blunted with DNA polymerase I, large (Klenow) fragment, and cloned into the blunted Cla I site just downstream of the CD3 promoter in the enhancer/promoter SK construct.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us