Figure 1
The effect of MP on IL-17 and IFN-γ gene expression and production in LNC. LNC isolated from DA rats (A, C-H) or AO rats (B) were stimulated with ConA (2.5 μg/ml) for 48 hours (A, B, E, F, H) or for various time periods (C, D) in the presence or absence of various MP concentrations (A, B), or with 10 ng/ml of MP (C-H). Subsequently, culture supernatants were collected for ELISA (A, B, C, D) or cells were collected for CFSE FACS analysis (H) or RNA isolation (E, F, G). Alternatively, cells were grown for additional 16 hours in the presence of 3H-thymidine for proliferation assay (A). Results are presented as % of values obtained in corresponding cultures without MP – control (A, B) or as concentrations (C, D), or as 2-dCt (E, F, G). CFSE staining profiles are: dark grey shadow – ConA, light grey line – ConA+MP, while the numbers in the table represent % of cells in corresponding phase. *p < 0.05 represents statistically significant difference to the corresponding culture without MP.