Figure 5

The effect of MP on MAPK signaling in ConA-stimulated LNC. A) LNC were stimulated with ConA (2.5 μg/ml) and incubated with or without MP (10 ng/ml) and/or ERK-inhibitor (UO126, 20 μM), or p38-inhibitor (SB202190, 20 μM), or Jnk-inhibitor (SP600125, 40 μM) for 24 hours. B) LNC cultivated with or without MP (10 ng/ml) for two hours and stimulated with ConA (2.5 μg/ml) for additional 40 minutes, were fixed and subjected to cELISA specific for phosphorylated ERK, p38, Jnk, Jun and c-Fos. cELISA results are presented as % of corresponding cultures without MP (control). *p < 0.05 represents statistically significant difference to the corresponding culture without MP.