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Fig. 1 | BMC Immunology

Fig. 1

From: IFNγ+ Treg in-vivo and in-vitro represent both activated nTreg and peripherally induced aTreg and remain phenotypically stable in-vitro after removal of the stimulus

Fig. 1

Determination of Treg subsets. a Total CD4+ lymphocytes of a healthy individual were gated (gate P2) and further analysed concerning intracellular Helios and IFNγ positivity. The majority of circulating CD4+ lymphocytes was Helios- and this gate was used for all further flow cytometric analyses of Helios positivity. b Stepwise gating strategy for Treg subset determination: first, lymphocytes gate (P1), then CD4+CD25+ PBL gate (P2), Foxp3+CD127- gate (P3), and finally Helios/IFNγ gate. Further CD4+CD25+Foxp3+CD127- Treg subsets (based on gate P3) were analysed using the depicted gate settings for IL10/TGFß, CXCR3/CD62L, CD252/CD152 (CD152 intracellular), CD119/Tbet, CD28/HLA-DR, CD152/CD154 (both surface), Perforin/GranzymeB, and CD95/CD178

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BMC Immunology

ISSN: 1471-2172

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