Fig. 1

Attenuated acute renal allograft rejection by EZH2 inhibition. Rat renal transplantation was performed as described in methods. Groups are classified in Methods. Renal grafts and recipient periphery blood were collected on day 5 after transplantation for analysis. (a) H&E and TUNEL staining were performed for renal grafts. (b) Histochemical quantitative data of Banff components including glomerulitis (g), tubulitis (t), interstitial inflammation (i), arteriola hyalinosis (ah), and intimal arteritis (v) was evaluated. (c) Semi-quantification apoptotic cells in renal graft parenchyma were examined. (d, e) EZH2 protein levels in peripheral CD3⁺CD25⁺ T cells were examined by western blot (d), and quantification was done by densitometry of western blot bands (normalized to actin) (e). *, p < 0.05, Kruskal-Wallis test