Fig. 1From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cellsKinetics of cytokine production. Milliplex kits from Millipore (see Material and Methods for details) were used to perform kinetic experiments with PBMC from two healthy donors stimulated for 1, 2 or 5 days with PPD (1 μg/ml) and SEB (10 ng/ml) as positive control. Non-stimulated (NS) PBMC were used as negative control. Results obtained for samples from donor 1 (IL-2 and IL-17) and donor 2 (IFNγ and IL-5) are shown. NS and PPD values correspond to the left y axis and SEB values to the right y axisBack to article page