Fig. 1From: MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesisProduction of cells in stromal co-cultures. Co-cultures were established by overlay of Lin− bone marrow over 5G3 stroma. (a) Hematopoietic cells adhered to stroma were observed under phase contrast microscopy at day 21. Bar represents 100 μm. (b) Gating procedure to detect CD11b+CD11c+ cells is shown for cells produced in co-cultures at 28-days. (c) Cell production was assessed at 14, 21 and 28 days using antibody staining and multicolor flow cytometry. Production of the CD11b−CD11c−, CD11b+CD11c−, CD11b+CD11c+, CD11b+CD11c+MHC-II−(L-DC) and CD11b+CD11c+MHC-II+ (cDC-like) subsets was calculated in terms of proportion of each subset amongst the total CD11b+ and/or CD11c+ population. Graphs show mean ± S.E. for triplicate co-cultures. Cell production in terms of subset size is statistically different (p ≤ 0.05) for days 21 and 28 compared with day 14 in terms of increased dendritic cell and reduced myeloid cell productionBack to article page