Fig. 2
From: Ionizing radiation modulates the phenotype and function of human CD4+ induced regulatory T cells
Radiation decreases Foxp3 expression in natural and induced human CD4 + CD25+ TREGS. nTREG and iTREG cells were mock irradiated or exposed to 10Gy of ionizing radiation. a After 48 h, nTREGS were stained for expression of CD4 and CD25 by flow cytometry. b Expression of Foxp3 was evaluated within the CD4 + CD25+ population. c 48 h after irradiation, TGF-β1-induced TREGS were stained for expression of CD4 and CD25 by flow cytometry. d Expression of Foxp3 was evaluated within the total CD4 + CD25+ population. e iTREGS were evaluated for the expression of Foxp3 within the CD4 + CD25hi population. Experiment was repeated three times with similar results. Error bars represent SEM. *P ≤ 0.05 by paired, one-tailed Student t test