Fig. 4

Correlation between proportions of Helios expressing Tregs (Helios⁺Tregs) and cytokine-producing CD8 T cells and seroreactivity to HIV-1 proteins. PBMC from PLWH were isolated and treated as described in the methods section. PBMC were stained with monoclonal antibodies and analyzed by polychromatic flow cytometry. The number of HIV-1 antigens, Env (gp160 and gp41), Gag (p24) and Pol (p31), recognized by antibodies, was analyzed on stored serum samples from all individuals living with HIV-1, using immuno-chromatographic test as described in Methods section. Viral loads were determined using plasma samples as described in the Methods section. Correlations between: a relative frequency of Helios expressing Tregs in PLWH-C and the proportion of CD8 T cells expressing IL-2 (n = 19). b proportions of Helios expressing Tregs and the number (#) of HIV-1 antigens recognized by antibodies for all PLWH (n = 24). Each data point corresponds to a single individual. Spearman’s correlation r and p-values are indicated on each figure